WebThe target sequence for the COX-2 siRNA was bases 291?C313 of NM000963.1. (5??-aactgctcaacaccggaattttt ... Tumor-associated macrophage-induced invasion and … WebJan 1, 2024 · The take up rate by an immortalized macrophage-like cell line of a number of mesoporous silica nanoparticles with differing diameter and pore size ... {Macrophage …
Did you know?
WebMar 28, 2024 · Iwata H, Goettsch C, Sharma A, Ricchiuto P, Goh WW, Halu A, Yamada I, Yoshida H, Hara T, Wei M, Inoue N, Fukuda D, Mojcher A, Mattson PC, Barabasi AL, … WebHere, macrophage‐like THP‐1 cells, exposed to ten different CNM for 48 h in low‐cytotoxic concentration of 10 µg mL−1, are characterized by secretion of ... epithelial type-II BEAS …
WebMar 3, 2024 · Briefly, for migration assay, 200 μL of cell suspensions without FBS (for THP-1 cells, 1 × 10 5 cells pretreated with 100 ng/mL PMA (Sigma-Aldrich, P8139) for 24 hours … WebJun 26, 2024 · Experiments were performed on human THP-1, a human monocyte-like cell line derived from a patient with leukaemia, obtained from ATCC bank (ATCC# ... Repnik U et al (2024) Co-culture of human alveolar epithelial (hAELVi) and macrophage (THP-1) cell lines. Altex 35:211–222. PubMed Google Scholar Latz E, Xiao TS , Stutz A (2013 ...
WebMar 30, 2024 · Tan AHY, Tu W, McCuaig R, Hardy K, Donovan T, Tsimbalyuk S, Forwood JK, Rao S. Lysine-Specific Histone Demethylase 1A Regulates Macrophage Polarization and … WebThe activity of AuNDs was evaluated using macrophage functions and antioxidant response in the human macrophage cell line THP-1. ... Nrf2 localizes within the cytoplasm and …
WebApr 24, 2024 · In parallel, THP-1 monocytes were differentiated into macrophage-like cells using PMA. When SMCs reached confluence ECs were seeded on the upper side of transwell inserts and allowed to adhere for 24 h. Inserts were then transferred into plates containing LPS stimulated adherent macrophage-like THP-1 cells.
WebSep 26, 2024 · The human monocytic THP-1 cells were cultured in RPMI media supplemented with 10% fetal bovine serum (FBS), 100 IU/ml penicillin, 100 μg/ml streptomycin, and 3.7 g/l sodium bicarbonate. The cells were maintained in 37°C temperature and 5% CO 2. 2.3. Differentiation of THP-1 Cells into Macrophage-Like Cells holiday homes davenport floridaWebJan 7, 2024 · We next assessed the functionality of NK-4-treated THP-1 macrophages by analyzing cytokine production. NK-4 dose-dependently increased THP-1 macrophage secretion of the proinflammatory cytokine TNF-α after 3 days of culture (Fig. 1b). At 4 μM NK-4, TNF-α secretion increased by 36-fold compared to control cells (Fig. 1b). NK-4 did … holiday homes county kerryWebThe invention discloses a construction method of a mononuclear macrophage model based on HIV-1 (human immunodeficiency virus type 1) mass infection duplication, which … holiday homes dingle kerry irelandWebMar 2, 2024 · In vitro studies using human monocytic cell lines such as THP-1, U937, MonoMac 6, ML-2 and HL-60 are frequently used to study monocyte/macrophage differentiation and function . The THP-1 used in this study are highly plastic and sensitive to many stimuli, and therefore can be polarized into multiple lineages (for review). huggingface sentencepieceWebThe invention discloses a construction method of a mononuclear macrophage model based on HIV-1 (human immunodeficiency virus type 1) mass infection duplication, which comprises the following steps: designing a pair of identification sequences for SAMHD1 gene CDS1, and constructing a TALEN plasmid pair; transfecting the TALEN plasmid pair … hugging face sentence embeddingWebAug 8, 2015 · Effects of IFN-γ and/or LPS on THP-1 macrophage viability after 16 or 24 h of incubation. THP-1 macrophages were incubated in RPMI medium with IFN-γ (20, 50 or … holiday homes down southWebInvivoGen offers several engineered THP-1 cell lines: THP1 NF-κB cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically … huggingface semantic search