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Csfv shimen strain

WebFeb 3, 2016 · Analysis of DGE libraries and annotation of unique tags. In this study, global gene expression profiles in SUVEC were analysed using the Solexa/Illumina DGE system to investigate the molecular changes following infection with CSFV C and Shimen strains. cDNA libraries from uninfected and CSFV (C and Shimen)-infected SUVEC were … WebJun 25, 2024 · The CSFV Shimen strain, a highly virulent strain isolated in China in 1945, were also used as a reference challenge virus in the present study. Primer design and RT-PCR. Six pairs of primers based on the published known sequence of CSFV Zj0801 (GenBank accession no. FJ529205) were designed for PCR analysis (Table ...

Tissue expression of Toll-like receptors 2, 3, 4 and 7 …

WebCSFV infection induced mitogen-activated protein kinase activation, but did not elicit NFκB activation, thereby affecting the production of pro-inflammatory cytokines. The Shimen … WebNov 29, 2012 · In the present study, CSFV Shimen strain full-length genomic cDNA carrying a genetic tag (AvaI site) was cloned downstream of the cytomegalovirus (CMV) promoter and T7 promoter to enable rescue by either of the methods described above. To ensure integral and precise transcription of the genome, the genomic cDNA was flanked … asa dns-guard https://gospel-plantation.com

Identification of E2 with improved secretion and …

WebMar 1, 2024 · The CSFV Shimen strain is a highly virulent strain isolated in China in 1945, which has been used as a reference challenge virus to evaluate the efficacy of CSF vaccines in China. The HLJZZ2014 strain is a subgenotype 2.1d CSFV isolated in 2014 in China (Luo et al., 2016). The CSFV Shimen and HLJZZ2014 strains were propagated … WebDocument: In a combined vaccine approach, SFV DNA vectors and recombinant adenovirus expressing the classical swine fever virus (CSFV) E2 glycoprotein elicited higher titers of neutralizing antibodies in pigs [26] . After challenges with the virulent CSFV Shimen strain, no symptoms of viremia were observed, for the combined vaccine, whereas vaccination … WebSep 8, 2014 · The virulent Shimen strain of CSFV, which is the standard virulent strain that has been used for vaccine potency tests in China since the 1950s, was used in this study. Prokaryotic expression of CSFV E2 and generation of … asad nasir jut md internal medicine

Coordinated expression of vascular endothelial growth factor A an…

Category:A comparison of the impact of Shimen and C strains of classical …

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Csfv shimen strain

Efficacy evaluation of the C-strain-based vaccines against the ...

Web猪瘟病毒野毒株E2蛋白特异性单抗免疫抑制制备法的建立.pdf WebNov 27, 2015 · The highly virulent CSFV Shimen strain (wt-Shimen) was used as a wild-type control in the experiment. EGFP-CSFV is an EGFP-tagged CSFV derived from pEGFP-CSFV [ 10 ]. vSM-HCLV3′UTR is a chimeric CSFV based on the wt-Shimen backbone with the 3′-UTR replacement by that of HCLV [ 8 ], which was generated in a way similar to …

Csfv shimen strain

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WebDuplex PCR for distinguishing shimen and C-strain of CSFV A duplex PCR for distinguishing the Shimen strain from the C-strain was performed with a total volume of … WebMay 1, 2024 · The chimeric CSFV/BVDV fragment consisted of the E2 gene of BVDV and p7 gene and incomplete NS2–3 coding region of CSFV, which was obtained from overlap PCR amplification using the cDNAs of BVDV Hubei strain and CSFV Shimen strain as templates. The pCSFV/bE2 mutants were constructed using genetic approaches based …

WebMar 13, 2024 · As shown in Fig. 4A,B, our method could detect the CSFV Shimen strain or the HCLV strain, while negative results were obtained … WebSep 8, 2014 · The virulent Shimen strain of CSFV, which is the standard virulent strain that has been used for vaccine potency tests in China since the 1950s, was used in this …

WebThe CSFV Shimen strain (GenBank accession number AF092248.2) was generated from a cDNA clone (pSPT I / SM) [18] and propagated in PK15 cells. The BVDV I Hubei strain (GenBank accession number ... WebNov 29, 2012 · In the present study, CSFV Shimen strain full-length genomic cDNA carrying a genetic tag (AvaI site) was cloned downstream of the cytomegalovirus (CMV) …

WebJul 1, 2015 · The Shimen strain infection resulted in a significant activation of IFN regulatory factor IRF7 and suppression of IRF3. ... two classical swine fever virus (CSFV) strains, namely the highly virulent CSFV Shimen strain and the avirulent C strain (a vaccine strain), were employed, and the expression of 19 immune effector genes was …

WebMar 1, 2024 · The CSFV Shimen strain is a highly virulent strain isolated in China in 1945, which has been used as a reference challenge virus to evaluate the efficacy of CSF … asad nasir mdWebApr 11, 2016 · The full-length cDNA of the CSFV Shimen strain, Alfort/187 strain or vaccine C-strain was amplified using the forward primer CSFV-F and the reverse primer CSFV-R. The PCR-amplified fragment was digested with Bsm BI and cloned into the Bsm BI sites of the pSPT I , yielding plasmid pSPT I /SM, pSPT I /187 or pSPT I /C. asad near meWebApr 23, 2014 · The CSFV Shimen and C strains were obtained from the Control Institute of Veterinary Bioproducts and Pharmaceuticals (Beijing, China). SUVECs were cultured in 25-cm 2 tissue culture flasks at a density of 2 × 10 7 cells per flask. The Shimen and C strains of CSFV were added to cultures at an MOI of 10 TCID50 per cell when SUVECs were … bangkok yanhee hospitalWebMay 31, 2024 · A full-length cDNA clone of attenuated strain HuN4-F112, obtained by serial cell passage from HP-PRRSV HuN4-F5 (GenBank accession no. EF635006), was used as the backbone for insertion of the E2 gene from the CSFV Shimen strain (GenBank accession no. AF092448) [24]. The resultant recombinant virus was compared to the … bangkok yaiWebNov 19, 2024 · Then, the cells were stimulated with ultraviolet-light-inactivated CSFV (Shimen strain; MOI = 1) l. After 24 h of incubation at 37 °C, the cell-free supernatant was removed and detected using a commercially available porcine IL-4 and IFN-γ ELISA kit according to the manufacturer’s protocol. OD values were measured at 450 nm using a ... a sad numberasado balkan grill beverungenWebClassical swine fever (CSF) caused by the classical swine fever virus (CSFV) has resulted in severe losses to the pig industry worldwide. It has been proposed that lipid synthesis is essential for viral replication, and lipids are involved in viral protein maturation and envelope production. However, the specific crosstalk between CSFV and host cell lipid metabolism … asa dobbing sunderland