WebFeb 3, 2016 · Analysis of DGE libraries and annotation of unique tags. In this study, global gene expression profiles in SUVEC were analysed using the Solexa/Illumina DGE system to investigate the molecular changes following infection with CSFV C and Shimen strains. cDNA libraries from uninfected and CSFV (C and Shimen)-infected SUVEC were … WebJun 25, 2024 · The CSFV Shimen strain, a highly virulent strain isolated in China in 1945, were also used as a reference challenge virus in the present study. Primer design and RT-PCR. Six pairs of primers based on the published known sequence of CSFV Zj0801 (GenBank accession no. FJ529205) were designed for PCR analysis (Table ...
Tissue expression of Toll-like receptors 2, 3, 4 and 7 …
WebCSFV infection induced mitogen-activated protein kinase activation, but did not elicit NFκB activation, thereby affecting the production of pro-inflammatory cytokines. The Shimen … WebNov 29, 2012 · In the present study, CSFV Shimen strain full-length genomic cDNA carrying a genetic tag (AvaI site) was cloned downstream of the cytomegalovirus (CMV) promoter and T7 promoter to enable rescue by either of the methods described above. To ensure integral and precise transcription of the genome, the genomic cDNA was flanked … asa dns-guard
Identification of E2 with improved secretion and …
WebMar 1, 2024 · The CSFV Shimen strain is a highly virulent strain isolated in China in 1945, which has been used as a reference challenge virus to evaluate the efficacy of CSF vaccines in China. The HLJZZ2014 strain is a subgenotype 2.1d CSFV isolated in 2014 in China (Luo et al., 2016). The CSFV Shimen and HLJZZ2014 strains were propagated … WebDocument: In a combined vaccine approach, SFV DNA vectors and recombinant adenovirus expressing the classical swine fever virus (CSFV) E2 glycoprotein elicited higher titers of neutralizing antibodies in pigs [26] . After challenges with the virulent CSFV Shimen strain, no symptoms of viremia were observed, for the combined vaccine, whereas vaccination … WebSep 8, 2014 · The virulent Shimen strain of CSFV, which is the standard virulent strain that has been used for vaccine potency tests in China since the 1950s, was used in this study. Prokaryotic expression of CSFV E2 and generation of … asad nasir jut md internal medicine